Single-Chromosome Micromanipulation Experiment
Marko Laboratory, Northwestern University
Field of view is approximately 100 microns.
First image is updated once per minute on this server, when
experiments are in progress.
This web page should refresh automatically.
Second image is from a previous experiment.
The objective of this experiment is to study the structure of the
mitotic chromosome, using unique biophysical techniques. We remove
single mitotic chromosomes from dividing cells using micropipettes.
Then, we suspend one chromosome between two pipettes, allowing us
to measure its elasticity via bending of one pipette.
In some experiments
we spray the chromosome with biochemicals which make specific
changes in its structure: we then observe changes via video
microscopy and force measurement.
This experiment has been developed by
Mike Poirier (UIC Ph.D. 2001),
Lisa Pope (UIC postdoctoral researcher 2003-2005),
Chee Xiong (UIC M.Eng. 2005),
Ryo Kawamura (UIC Ph.D. student 2005-2008),
Mingxuan Sun (NU IBiS Ph.D. student, 2007-2013),
Jessica Hornick (NU postdoctoral researcher 2012-2016),
Andrew Stephens (NU postdoctoral researcher, 2013-2019),
and Ronald Biggs (NU IBiS Ph.D. student, 2014-present).
Supported by National Science Foundation Grants
MCB-0240998 and MCB-1022117,
and National Institutes of Health Grants
U54-HD076188 (NU Center for Reproductive Research After Disease),
and a subcontract to U54-DK107980 (4D Nucleome).
Marko Lab PubMed Publication List