Single-Chromosome Micromanipulation Experiment
Marko Laboratory, Northwestern University
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Experiments are done most weekdays, usually in the early afternoon.

The objective of this experiment is to study the structure of the mitotic chromosome, using unique biophysical techniques. We remove single mitotic chromosomes from dividing cells using micropipettes. Then, we suspend one chromosome between two pipettes, allowing us to measure its elasticity via bending of one pipette. In some experiments we spray the chromosome with biochemicals which make specific changes in its structure: we then observe changes via video microscopy and force measurement.

This experiment was developed by Mike Poirier (UIC Ph.D. 2001), Lisa Pope (UIC postdoctoral researcher 2003-2005), Chee Xiong (UIC M.Eng. 2005), Ryo Kawamura (UIC Ph.D. student 2005-2008), Mingxuan Sun (Northwestern Ph.D. student, 2007-2013), Jessica Hornick (NU postdoctoral researcher 2012-present), and Andrew Stephens (NU postdoctoral researcher, 2013-present).

Supported by National Science Foundation Grants MCB-0240998 and MCB-1022117,
and National Institutes of Health Grants 1U54CA143869-01 (NU-PS-OC),
1U54HD076188 (NU Center for Reproductive Research After Disease), and 1R01GM105847-01.
Marko Lab PubMed Publication List