| name | size (Mb) |
description
and citation if published (current PubMed publications) |
| alu1.mpg |
4.5 |
digestion of single newt mitotic
chromosome using ~1 nM AluI restriction enzyme, demonstrating that
well-spaced DNA cuts are sufficient to fully disconnect a mitotic
chromosome (Poirier and Marko PNAS 2002) |
| assmb-dimer-4fs-1p5-short.avi |
5.4 |
lambda DNA dimer (97 kb) being
assembled into a chromatin fiber using 1/20 dilution of Xenopus egg
extracts, against 1.5 pN applied force (Yan et al MBC 2007) |
| chromlow.mpg |
0.5 |
single native newt chromosome
undergoing reversible stretch-retract cycle (Poirier and Marko MBC 2000) |
| cut-thin-fiber.mpg |
2.9 |
mitotic newt chromosome, after
being lightly digested with micrococcal nuclease and then stretched
out, is cut by a single additional puff of mc nuclease (Poirier and Marko PNAS 2002) |
| dimer-4fs-1p5.avi |
1.8 |
lambda DNA dimer (97 kb) in
buffer held by 1.5 pN constant force in transverse magnetic tweezer
experiment, undergoing small fluctuations (Yan et al PRE 2004; Yan et al MBC 2007) |
| fluct.avi |
19.7 |
magnetic bead suspended by an
individual lambda DNA (48.5 kb) attached to a cover slip, viewed in
vertical magnetic tweezer setup (Skoko et al Biochemistry 2004) |
| hse_assemb_2fs.avi |
18.4 |
lambda DNA dimer (97 kb) being
assembled into a chromatin fiber using
1/20 dilution of Xenopus egg extracts, against 1.5 pN applied force
(Yan et al MBC 2007) |
| lambda+0-052004.avi |
1.9 |
magnetic bead suspended by an
individual lambda DNA (48.5 kb) attached
to a cover slip, viewed in vertical magnetic tweezer setup ; bead is
being pulled by approximately 0.05 pN and is about 4 microns below
surface (Skoko et al Biochemistry 2004) |
| lambda+200-052004.avi |
1.2 |
magnetic bead suspended by an
individual lambda DNA (48.5 kb) attached
to a cover slip, viewed in vertical magnetic tweezer setup; bead is
being pulled by approximately 0.1 pN and is about 8 microns below cover
slip surface (Skoko et al Biochemistry 2004) |
| lambda+370-052004.avi |
1.2 |
magnetic bead suspended by an
individual lambda DNA (48.5 kb) attached
to a cover slip, viewed in vertical magnetic tweezer setup ; bead is
being pulled by approximately 10 pN and is about 16 microns (the full
length of the DNA) below the cover slip surface (Skoko et al Biochemistry 2004) |
| mg100.mpg |
0.2 |
single mitotic newt chromosome
being sprayed by 100 mM MgCl in tissue culture buffer; note
reversibility of the ionic-screening-driven transition (Poirier et al J. Cellular Biochem. 2002) |
| micrococ1.mpg |
4.3 |
single mitotic newt chromosome
being sprayed by ~1 nM micrococcal nuclease, which cuts DNA at all
sequence sites; chromosome is disconnected and disintegrates into
apparent 'droplet' of chromatin fragments (Poirier and Marko PNAS 2002) |
| micrococ2.mpg |
2.9 |
single mitotic chromosome after
mild micrococcal nuclease treatment so that morphology is not changed
at zero force; as it is stretched, it breaks into islands of dense
chromatin connected by thin fibers (Poirier and Marko PNAS 2002) |
| mitosis.mpg |
90.1 |
mitosis in a newt lung cell; the
'hiccups' are caused by the microscope focusing through the cell
periodically |
| triton.mpg |
38.0 |
extraction of a single mitotic
chromosome from a newt cell in mitosis; Triton X-100 0.05% by volume is
used in the first pipette you see, to open a hole in the cell |
| Bajer-mitosisIII.avi |
16.0 |
cell division in fragment of plant cell showing passage of linked circular chromosomes through one another, as described in A. Bajer, Chromosoma 14, 18-30 (1963) |